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KMID : 0624620120450100571
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BMB Reports
2012 Volume.45 No. 10 p.571 ~ p.576
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X-ray radiation at low doses stimulates differentiation and mineralization of mouse calvarial osteoblasts
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Park Soon-Sun
Kim Kyoung-A
Lee Seung-Youp
Lim Shin-Saeng
Jeon Young-Mi
Lee Jeong-Chae
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Abstract
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Radiotherapy is considered to cause detrimental effects on bone tissue eventually increasing bone loss and fracture risk. However, there is a great controversy on the real effects of irradiation itself on osteoblasts, and the mechanisms by which irradiation affects osteoblast differentiationand mineralization are not completely understood. We explored how X-ray radiation influencesdifferentiation and bone-specific gene expression in mouse calvarial osteoblasts. Irradiation at 2 Gy not only increased differentiation and mineralization of the cells, but also upregulated the expression of alkaline phosphatase, type I collagen, osteopontin, and osteocalcin at early stages of differentiation. However, irradiation at higher doses (>2 Gy) did not stimulate osteoblastdifferentiation, rather it suppressed DNA synthesis by the cells without a toxic effect. Additional experiments suggested that transforming growth factor-beta 1 and runt-transcription factor 2 play important roles in irradiation- stimulated bone differentiation by acting as upstream regulators of bone-specific markers.
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KEYWORD
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Bone-formation regulatory factors, Ionizing irradiation, Mineralization, Mouse calvarial osteoblasts, TGF-β1
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